Strategic improvement of Shewanella oneidensis for biocatalysis: approach to media refinement and scalable application in a microbial electrochemical system

Publikationstyp
Journal Article
Date Issued
2024-11-28
Sprache
English
Author(s)
Jürgensen, Nikolai 
Bioprozess- und Biosystemtechnik V-1  
Weiler, Janek 
Technische Mikrobiologie V-7  
Knoll, Melanie Tabea 
Technische Mikrobiologie V-7  
Gescher, Johannes  
Technische Mikrobiologie V-7  
Edel, Miriam 
Technische Mikrobiologie V-7  
TORE-DOI
10.15480/882.14159
TORE-URI
https://tore.tuhh.de/handle/11420/52786
Journal
New biotechnology  
Volume
85
Start Page
31
End Page
38
Citation
New Biotechnology 85: 31-38 (2025-03-25)
Publisher DOI
10.1016/j.nbt.2024.11.006
Scopus ID
2-s2.0-85211063521
Publisher
Elsevier
Peer Reviewed
true
Microbial electrochemical systems offer a sustainable method for the conversion of chemical energy into electrical energy or hydrogen and the production of valuable compounds, contributing to the development of a bio-based economy. This study aimed to enhance the performance of anodic bioelectrochemical systems by improving the current density of Shewanella oneidensis as a biocatalyst through strain modification and medium refinement. The genetic modification, combining the prophage deletion and overexpression of the speC gene, resulted in a 4.2-fold increase in current density compared to the wild type. Furthermore, medium refinement and incorporating riboflavin, led to an additional 5.7-fold increase in current density. The application of the modified strain and medium in a scalable microbial electrolysis cell resulted in a current density of 1.2 A m-², similar to what was achieved previously with an S. oneidensis and Geobacter sulfurreducens co-culture, substantiating the substantial performance increase for a pure culture of S. oneidensis. Furthermore, S. oneidensis was shown to grow in medium containing up to 500 mM sodium chloride and increasing the salt concentration to 400 mM had a minor influence on growth but significantly lowered the cell voltage of the MEC system.
Subjects
Bioelectrochemical system | Current density | Microbial electrolysis cell | Scale-up | Shewanella oneidensis
DDC Class
660.6: Biotechnology
Publication version
publishedVersion
Lizenz
https://creativecommons.org/licenses/by-nc-nd/4.0/
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