Evaluation of deep eutectic solvents as alternative reaction media in biocatalysis
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Enzyme catalysis is often associated with aqueous reaction media, but water is rather an inadequate solvent for hydrophobic substrates. In this study ‘deep eutectic solvents’ (DESs) were evaluated as novel reaction media for biocataylsis. For instance, a DES composed of both substrates, i.e. a DES made of (–)-Menthol:lauric acid, was used as combined solvent and substrate for the lipase catalysed synthesis of (–)-menthyl laurate, so that no additional solvent was necessary. The optimisation of the reaction resulted in a batch productivity of 443 g L−1 d−1 at 95 % conversion (24 h). Excess (–)-menthol was separated in a vacuum distillation step and was reused for a second enzymatic DES reaction.
Deep eutectic solvents
570: Biowissenschaften, Biologie