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Evaluation of deep eutectic solvents as alternative reaction media in biocatalysis
Citation Link: https://doi.org/10.15480/882.2990
Publikationstyp
Doctoral Thesis
Date Issued
2020-09
Sprache
English
Author(s)
Advisor
Referee
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2020-06-12
Institut
TORE-DOI
TORE-URI
First published in
Number in series
21
Citation
Schriftenreihe des DECHEMA-Forschungsinstituts 21: (2020)
Publisher
Shaker Verlag
Enzyme catalysis is often associated with aqueous reaction media, but water is rather an inadequate solvent for hydrophobic substrates. In this study ‘deep eutectic solvents’ (DESs) were evaluated as novel reaction media for biocataylsis. For instance, a DES composed of both substrates, i.e. a DES made of (–)-Menthol:lauric acid, was used as combined solvent and substrate for the lipase catalysed synthesis of (–)-menthyl laurate, so that no additional solvent was necessary. The optimisation of the reaction resulted in a batch productivity of 443 g L−1 d−1 at 95 % conversion (24 h). Excess (–)-menthol was separated in a vacuum distillation step and was reused for a second enzymatic DES reaction.
Subjects
Biocatalysis
Deep eutectic solvents
Lipase
Menthol
Esterification
DDC Class
500: Naturwissenschaften
570: Biowissenschaften, Biologie
600: Technik
Publication version
publishedVersion
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Dissertation_MP.pdf
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1.8 MB
Format
Adobe PDF