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    Item type:Research Data, Access status: with files ,
    Dataset - Gyroid structured Globugraphite carbon foam with hierarchical and tailored pore size
    (2026-04-30)
    Roller, Christiane
    Ordered polymer printed sacrificial templates are employed to get tailored carbon foam structures called Globugraphite for electrode applications. The Globugraphite synthesis is based on the carbon replication of a porous zinc oxide template while simultaneously reducing the template material in the chemical vapor deposition by hydrogen etching introduced by Marx et al., Previously Polyvinylbutyral filler was used to create a statistical distribution of pores. In order to create additional macropores up to 1000µm in the ceramic template additive manufactured nets and lattices are used to imprint an ordered pore network. Besides Poly Lactic Acid, a lightweight foaming filament is used to reduce internal stresses during sintering. Additive manufacturing proves as a viable procedure to imprint interconnected networks with defined macropores into Globugraphite. The pore size distribution is analyzed via mercury intrusion and morphology changes by scanning electron microscopy. Based on these analysis methods a clear correlation between the structure of the lattice used for template imprint and imprint in Globugraphite can be observed. Furthermore, the thermal stability of Globugraphite have been analyzed via thermogravimetric analysis. Individual tailoring of the pore size distribution and the overall morphology of Globugraphite are accomplished.
    Data Type: Dataset
      48  74
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    Identification of genetic determinants that promote biofilm growth under heterotrophic conditions in Cupriavidus necator using transposon enrichment
    (2026-07-06)
    Weiler, Janek
    These datasets contain measurements of OCT and a crystal violet assay to compare biofilm formation of two different Cupriavidus necator strains.
    Data Type: Experimental Data
      17  27
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    Item type:Research Data, Access status: with files ,
    Dataset - Effect of hydrocarbon precursor on morphology and properties of Globugraphite Carbon Foam
    (2026-10-05)
    Roller, Christiane
    This study investigates the effect of hydrocarbon precursors on the morphology and properties of Globugraphite, a porous carbon foam, in order to achieve scalable, tailored functionalizations. This dataset contains both microscopy images and experimental measurement data.
    Data Type: Dataset ; Data Type: Still Image
      14  59
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    Item type:Research Data, Access status: with files ,
    Training Cupriavidus necator for growth on cathode surfaces: impact of adaptive evolution for genomic alterations leading to accelerated biofilm development
    (2026-09-23)
    Chavali, Sri Sannihita
    Cupriavidus necator is a highly adaptable chemolithoautotroph and a promising chassis for sustainable bioproduction from CO₂. Coupling its metabolism to renewable electricity through microbial electrosynthesis (MES) requires the organism to form stable, electroactive biofilms on cathodes. This step is hindered under oxic conditions (oxic MES, oMES) by electrochemically generated reactive oxygen species (ROS), mass-transfer limitations and poor biofilm stability. Systematic strategies to improve C. necator biofilm formation on cathodes have been lacking. Here we used adaptive laboratory evolution (ALE) to address this gap. The progenitor strain colonised graphite cathodes only after an initial heterotrophic phase and formed spatially uneven biofilms whose distribution mirrored the local flow field, as resolved by micro-particle tracking velocimetry. Populations were then selected over five passages under controlled, oxygen-limited cathodic conditions. Resequencing revealed that the recovered population was genetically heterogeneous, and its dominant isolate, strain 890, was characterised in a microfluidic oMES platform. Strain 890 reached the target electrode coverage in about one day instead of six and maintained a robust biofilm at the progenitor's steady-state biovolume, whereas the multi-strain pool peaked higher but proved unstable. Whole-genome sequencing linked this accelerated, biofilm-forward phenotype to only two loci, both potentially central regulators of the planktonic-to-biofilm transition. The steady-state biovolume itself remained bounded most probably due to a mass-transfer ceiling set by the counter-directional supply of H₂ and O₂. This work shows that ALE renders the initial-attachment barrier of cathodic biofilm formation genetically tractable, providing strain 890 as a robust chassis and a well-defined starting point for oMES-based CO₂ valorisation.
    Data Type: Dataset
      10  40
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    Item type:Research Data, Access status: with files ,
    Aerographite vs. Graphite: Investigating the Role of Electrode Architecture in H₂-Mediated Electroautotrophic Biofilms
    (2026-03-05)
    Chavali, Sri Sannihita
    This study investigates biofilm development by 𝘊𝘶𝘱𝘳𝘪𝘢𝘷𝘪𝘥𝘶𝘴 𝘯𝘦𝘤𝘢𝘵𝘰𝘳 H16 on Aerographite, an ultralow-density carbon foam (3-6 mg cm⁻³, porosity >99%) with a hierarchical tetrapodal pore network, as a cathode material in microfluidic bioelectrochemical systems and compares it to plain graphite.
    Data Type: Dataset
      75  134