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Item type:Publication, Access status: with files , Integration of the all-in-one electrode in an electrochemical flow cell for in situ hydrogen peroxide supply in hydroxylation mediated by immobilized unspecific peroxygenase(Elsevier, 2025-08-01); ; ; ; Hydrogen peroxide (H₂O₂) is a strong oxidizing agent that is commonly employed in chemical synthesis. Nevertheless, its utilization as a cosubstrate in biocatalytic reactions remains limited due to the deactivating effect on biocatalysts at an elevated concentration. An electrochemical synthesis of H₂O₂ represents an attractive approach, offering a controllable in situ generation of H₂O₂ without producing complex by-products. The objective of this study is to demonstrate the feasibility of the in situ electrogeneration of H₂O₂ using the All-in-One (AiO) electrode within a flow reactor technology. Integrating a bioelectrochemical system (BES) into a flow reactor technology, such as a flow cell, presents an alternative strategy for scale-up. In this study, the in situ generation of H₂O₂ is coupled with the hydroxylation of 4-ethylbenzoic acid catalyzed by the immobilized recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO) within a complete BES under batch and fed-batch operation modes. The electrochemical flow cell facilitates a controllable H₂O₂ generation by adjusting experimental parameters such as current density, aeration rate and residence time. The flow cell BES equipped with the AiO electrode yielded a catalytic productivity as high as 1.24 ± 0.02 mM h−1 (4.95 ± 0.1 g L−1 d−1), a total turnover number of rAaeUPO up to 3.38 · 105 ± 702 mol mol−1 and a turnover frequency up to 8.34 ± 0.14 s−1.Publicationtype: Journal Article55 47 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Understanding the copper underpotential deposition process at strained gold surfaceCopper underpotential deposition (UPD) on a gold surface is investigated by cyclic voltammetry coupled with in situ cyclic strain to understand the strain-modulated electrodeposition. Our work emphasizes quantification of an electrocapillary coupling coefficient ς, which relates the response of Cu electrodeposition potential, E, to applied strain, ε. The different responses to the strain are observed at two Cu UPD stages. The data indicate that tensile strain could enhance the formation of a Cu monolayer on the Au surface. The typical electrodeposition process could be modulated by an external mechanical strain.Publicationtype: Journal Article98