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    Item type:Publication, Access status: without files ,
    On-Chip Neo-Glycopeptide Synthesis for Multivalent Glycan Presentation
    (2020-08-06)
    Mende, Marco
    ;
    Tsouka, Alexandra
    ;
    Heidepriem, Jasmin
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    Paris, Grigori
    ;
    Mattes, Daniela S.
    Single glycan–protein interactions are often weak, such that glycan binding partners commonly utilize multiple, spatially defined binding sites to enhance binding avidity and specificity. Current array technologies usually neglect defined multivalent display. Laser-based array synthesis technology allows for flexible and rapid on-surface synthesis of different peptides. By combining this technique with click chemistry, neo-glycopeptides were produced directly on a functionalized glass slide in the microarray format. Density and spatial distribution of carbohydrates can be tuned, resulting in well-defined glycan structures for multivalent display. The two lectins concanavalin A and langerin were probed with different glycans on multivalent scaffolds, revealing strong spacing-, density-, and ligand-dependent binding. In addition, we could also measure the surface dissociation constant. This approach allows for a rapid generation, screening, and optimization of a multitude of multivalent scaffolds for glycan binding.
    Publicationtype: Journal Article
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    Item type:Publication, Access status: without files ,
    Multivalent glycan arrays
    (2019-01-01)
    Mende, Marco
    ;
    Bordoni, Vittorio
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    Tsouka, Alexandra
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    Loeffler, Felix F.
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    Delbianco, Martina
    Glycan microarrays have become a powerful technology to study biological processes, such as cell-cell interaction, inflammation, and infections. Yet, several challenges, especially in multivalent display, remain. In this introductory lecture we discuss the state-of-the-art glycan microarray technology, with emphasis on novel approaches to access collections of pure glycans and their immobilization on surfaces. Future directions to mimic the natural glycan presentation on an array format, as well as in situ generation of combinatorial glycan collections, are discussed.
    Publicationtype: Journal Article
      4