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Item type:Publication, Access status: with files , Development of an experimental setup and a validated model for a highly integrated biocatalytic process(American Chemical Society (ACS), 2026-07-23); ; ; ; The development and validation of a mathematical model for a highly integrated biocatalytic process are presented. A multienzymatic cascade comprising three parallel and sequential reaction steps was implemented in a miniplant setup, integrating an enzyme membrane reactor (EMR) and a reactive extraction centrifuge (REC) to enable intensified biotransformations. Laboratory-scale experiments were conducted to obtain the kinetic and thermodynamic data necessary for model development. The resulting process model captures both the enzymatic reaction kinetics and the dynamic behavior of the integrated unit operations. Model validation was performed under representative process conditions in the miniplant system. The results demonstrate good agreement between the model and experimental data, confirming the predictive power of the model and supporting its use in future process design, scale-up, and optimization. This work underscores the importance of model-based development for advancing sustainable and efficient biocatalytic processes.Publicationtype: Journal Article27 9 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Debottlenecking a 2-phase multi-enzymatic cascade by an enzyme membrane reactor – Modelling and experimental validation(Elsevier, 2025-08-08); ; ; ; Biotechnological processes have a high potential to make industrial processes more sustainable. However, biotechnological processes often have low product concentrations and production rates. This is where process intensification can help to make these processes competitive. Using the example of the multi-enzymatic synthesis of natural cinnamyl cinnamate, it is shown how an existing multi-enzymatic process can be intensified by using an enzyme membrane reactor. The individual enzymatic reactions are first characterised in laboratory scale and then combined in a mini plant. In addition, a process model of the mini plant is developed. It is shown that the use of an enzyme membrane reactor can increase the production rate of the multi-enzymatic process by a factor of 10 compared to a previously investigated set-up.Publicationtype: Journal Article57 22 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Analytical and numerical approaches to the analysis of progress curves: a methodological comparisonAccurate models of the reaction kinetics of enzymatic reactions are essential for the design of biocatalytic processes. While many experimental studies still build on initial slope analysis, progress curve analysis offers the potential for modelling enzymatic reactions with a significantly lower experimental effort in terms of time and costs, but requires the solution of a dynamic nonlinear optimization problem. There are many different approaches for solving this problem for parameter regression, building on the experimental progress curve data. In order to provide some guidance for selecting an appropriate approach, this study presents a detailed comparison of two analytical and two numerical approaches analysing their strengths and weaknesses on the basis of three case studies. The analytical approaches build on the implicit and explicit integrals of the respective reaction rate equations, while the numerical approaches consider the direct numerical integration of the differential mass balance equations as well as the transformation of the dynamic problem to an algebraic problem by means of spline interpolation of the reaction data. In particular, the dependence of the results on the initial parameter estimates is evaluated, showcasing that the numerical solution with spline interpolation shows a lower dependence on the initial values providing parameter estimates comparable to the analytical approaches, which are however limited in applicability.Publicationtype: Journal Article47 17 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Concept of an enzymatic reactive extraction centrifuge(Multidisciplinary Digital Publishing Institute (MDPI), 2022-10-20); ; ; Biocatalytic processes often provide an ecological alternative to many chemical processes. However, further improvements in terms of the economic efficiency are required. In order to achieve that, the concept of process integration is a promising option. Applying this within a biocatalytic process, a highly integrated apparatus working as a reactive extraction centrifuge was developed and operated. For this purpose, a commercially available extraction centrifuge was modified to implement a biocatalytic reaction. The novel apparatus was used within a multi-enzyme cascade for the production of a natural flavor and fragrance, namely cinnamic ester. The characterization of the reactive extraction centrifuge and the suitable operation conditions for the inlet streams and the rotational speed for a stable operation were determined. Furthermore, different initial substrate concentrations were applied to prove the reaction. The results provide a successful proof of concept for the novel reactive extraction centrifuge.Publicationtype: Journal ArticleScopus© Citations 1 126 281 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Commissioning and operation of an enzymatic reactive extraction centrifuge.Publicationtype: Conference Poster not in Proceedings183 135 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Multi-enzyme cascade reaction in a miniplant two-phase-system: model validation and mathematical optimization(Wiley, 2021-01-11); ; ; ; Biotechnological application of multiple enzymes in different phases for target compounds synthesis poses a significant challenge for industrial process development. At the same time, a growing demand for natural flavors and fragrances opens up possibilities for novel biotechnological processes to replace current chemical synthesis routes, with additional advantages such as avoiding harsh reaction conditions and toxic chemicals, and less by-products in the system. Within complex biotechnological processes, the key for unfolding their industrial application potential in bioprocess engineering lies in their mathematical modeling. In this contribution, a multi-enzyme cascade reaction in a two-phase system implemented in a miniplant-scale reactor setup is mathematically modeled for the example of the flavoring agent cinnamyl cinnamate. Using our validated model and a mathematical optimization tool based on a genetic algorithm, optimization runs are performed to demonstrate the potential of computer-aided process development for complex biotechnological processes.Publicationtype: Journal ArticleScopus© Citations 11 157 338 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Process for the preparation of fatty aldehydes from renewable resourcesCommon methods for the chemical production of fatty aldehydes, widely applied as artificial flavors and fragrances, use fossil starting materials, harsh reaction conditions, and hazardous chemicals. This contribution introduces a novel, environmentally friendly process for the preparation of fatty aldehydes from the natural, renewable resource plant oil. It has successfully been shown that bringing a fatty acid into contact with zerovalent iron leads to the reduction of the fatty acid to the corresponding fatty aldehyde. In this paper, the three-step process is presented and discussed, exemplary for the preparation of dodecylaldehyd from dodecanoate.Publicationtype: Journal ArticleScopus© Citations 7 191 250