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Item type:Publication, Access status: without files , Systematically Designed Periodic Electrophoretic Deposition for Decorating 3D Carbon-Based Scaffolds with Bioactive NanoparticlesThe coating of porous scaffolds with nanoparticles is crucial in many applications, for example to generate scaffolds for catalysis or to make scaffolds bioactive. A standard and well-established method for coating surfaces with charged nanoparticles is electrophoresis, but when used on porous scaffolds, this method often leads to a blockage of the pores so that only the outermost layers of the scaffolds are coated. In this study, the electrophoretic coating process is monitored in situ and the kinetics of nanoparticle deposition are investigated. This concept can be extended to design a periodic electrophoretic deposition (PEPD) strategy, thus avoiding the typical blockage of surface pores. In the present work we demonstrate successful and homogeneous electrophoretic deposition of hydroxyapatite nanoparticles (HAn, diameter ≤200 nm) on a fibrous graphitic 3D structure (ultralightweight aerographite) using the PEPD strategy. The microfilaments of the resulting scaffold are covered with HAn both internally and on the surface. Furthermore, protein adsorption assays and cell proliferation assays were carried out and revealed that the HAn-decorated aerographite scaffolds are biocompatible. The HAn decoration of the scaffolds also significantly increases the alkaline phosphatase activity of osteoblast cells, showing that the scaffolds are able to promote their osteoblastic activity.Publicationtype: Journal Article196 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Biomimetic Carbon Fiber Systems Engineering: A Modular Design Strategy To Generate Biofunctional Composites from Graphene and Carbon Nanofibers(2019-02-06); ; ; ; Carbon-based fibrous scaffolds are highly attractive for all biomaterial applications that require electrical conductivity. It is additionally advantageous if such materials resembled the structural and biochemical features of the natural extracellular environment. Here, we show a novel modular design strategy to engineer biomimetic carbon fiber-based scaffolds. Highly porous ceramic zinc oxide (ZnO) microstructures serve as three-dimensional (3D) sacrificial templates and are infiltrated with carbon nanotubes (CNTs) or graphene dispersions. Once the CNTs and graphene coat the ZnO template, the ZnO is either removed by hydrolysis or converted into carbon by chemical vapor deposition. The resulting 3D carbon scaffolds are both hierarchically ordered and free-standing. The properties of the microfibrous scaffolds were tailored with a high porosity (up to 93%), a high Young's modulus (ca. 0.027-22 MPa), and an electrical conductivity of ca. 0.1-330 S/m, as well as different surface compositions. Cell viability, fibroblast proliferation rate and protein adsorption rate assays have shown that the generated scaffolds are biocompatible and have a high protein adsorption capacity (up to 77.32 ± 6.95 mg/cm3) so that they are able to resemble the extracellular matrix not only structurally but also biochemically. The scaffolds also allow for the successful growth and adhesion of fibroblast cells, showing that we provide a novel, highly scalable modular design strategy to generate biocompatible carbon fiber systems that mimic the extracellular matrix with the additional feature of conductivity.Publicationtype: Journal Article181 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , A tunable scaffold of microtubular graphite for 3D cell growth(Soc., 2016-06-03); ; ; ; Aerographite (AG) is a novel carbon-based material that exists as a self-supportive 3D network of interconnected hollow microtubules. It can be synthesized in a variety of architectures tailored by the growth conditions. This flexibility in creating structures presents interesting bioengineering possibilities such as the generation of an artificial extracellular matrix. Here we have explored the feasibility and potential of AG as a scaffold for 3D cell growth employing cyclic RGD (cRGD) peptides coupled to poly(ethylene glycol) (PEG) conjugated phospholipids for surface functionalization to promote specific adhesion of fibroblast cells. Successful growth and invasion of the bulk material was followed over a period of 4 days.Publicationtype: Journal ArticleScopus© Citations 24 295 405