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Item type:Publication, Access status: with files , Selective bioelectrochemical purification of propionate from food waste hydrolysate(Elsevier, 2026-12-21); ; Dark fermentation enables hydrogen production while generating propionate-rich streams from food waste. However, efficient separation of propionate from short-chain fatty acid (SCFA) mixtures remains challenging and costly in the downstream processes. Therefore, strategies enabling selective purification of propionate prior to separation from the fermentation broth are highly desirable. In this study, a bioelectrochemically assisted bench-scale process was developed as a proof-of-concept pretreatment to enhance selective purification of propionate prior to downstream processing. A single-chamber rotating disc bioelectrochemical reactor (RDBER) was used to selectively purify the hydrolysate via enhancing the consumption of butyrate by an electroactive biofilm of a recently published butyrate-oxidizing microbial consortium. In the RDBER, different hydraulic retention times (HRTs) without and with pH control were evaluated to investigate their effects on propionate fraction among SCFAs and process stability. The results show that with a pH of 7.0–7.3 and an HRT of 8 days, a propionate fraction of up to 95.5% can be achieved, with its concentration doubling compared to the feed. These results demonstrate the potential of this process as a pretreatment step prior to downstream separation, with the aim of reducing separation complexity and enhancing propionate recovery.Publicationtype: Journal Article16 7 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Design approach and performance of a scalable granular packed bed biofilter for electro-fermentationBioelectrochemical systems (BES) represent a promising technology for the production of energy, energy carriers, and chemicals; however, their implementation in industrial applications faces significant challenges related to scalability. In this study, we introduce a novel, scalable, single-chamber BES in which the working electrode is realised as a packed bed of electrically conductive equal-sized spheres made from a graphite-polymer composite. The reactor has a total volume of 3.56 L and a packed bed volume of 1.66 L with an electrode surface area of 1.06 m². A model microbial electrolysis / electro-fermentation of lactate to acetate was catalysed by the exoelectrogenic bacterium Shewanella oneidensis , achieving a maximum current density of 430 mA m<sup>-2</sup> at an anode potential of 300 mV relative to the standard hydrogen electrode.Publicationtype: Journal Article19 6 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Efficiency and process development for microbial biomass production using oxic bioelectrosynthesis(Elsevier, 2025-03); ; ; ; Rosa, Luis F. M.Autotrophic microbial electrosynthesis (MES) processes are mainly based on organisms that rely on carbon dioxide (CO2) as an electron acceptor and typically have low biomass yields. However, there are few data on the process and efficiencies of oxic MES (OMES). In this study, we used the knallgas bacterium Kyrpidia spormannii to investigate biomass formation and energy efficiency of cathode-dependent growth. The study revealed that the process can be carried out with the same electron efficiency as conventional gas fermentation, but overcomes disadvantages, such as the use of explosive gas mixtures. When accounting only for the electron input via electrical energy, a solar energy demand of 67.89 kWh kg–1 dry biomass was determined. While anaerobic MES is ideally suited to produce methane, short-chain alcohols, and carboxylic acids, its aerobic counterpart could extend this important range of applications to not only protein for use in the food and feed sector, but also further complex productsPublicationtype: Journal Article24 22