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    Item type:Publication,
    Confined Flash Printing and Synthesis of Stable Perovskite Nanofilms under Ambient Conditions
    (2024-11-14) ;
    Knaus, Tanja
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    ; ;
    The fabrication of stable perovskite nanofilm patterns is important for the development of functional optical devices. However, current production approaches are limited by the requirement for strict inert gas protection and long processing times. Here, a confined flash printing synthesis method is presented to generate perovskite nanofilms under ambient conditions, combining precursor transfer, perovskite synthesis, crystallization, and polymer protection in a single step within milliseconds. A laser simultaneously prints and induces the flash synthesis, confined in a polymer nanofilm, under normal ambient conditions. Due to its simplicity and flexibility, the method enables the combination and screening of many different perovskite precursor materials on various substrates. Besides for the development of novel perovskite materials and devices, the nanofilms can be applied for biodetection. The unique H<inf>2</inf>O<inf>2</inf>-responsive property of the ultrathin perovskite quantum dot film is applied for biomolecule detection based on oxidase-catalyzed enzymatic reactions.
    Publicationtype: Journal Article
      12
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    Item type:Publication,
    Recyclable and Robust Optical Nanoprobes with Engineered Enzymes for Sustainable Serodiagnostics
    (2023-11-23)
    Liu, Yuxin
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    ; ;
    Zhu, Xingjun
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    Knaus, Tanja
    Recyclable fluorescence assays that can be stored at room temperature would greatly benefit biomedical diagnostics by bringing sustainability and cost-efficiency, especially for point-of-care serodiagnostics in developing regions. Here, a general strategy is proposed to generate recyclable fluorescent probes by using engineered enzymes with enhanced thermo-/chemo-stability, which maintains an outstanding serodiagnostic performance (accuracy >95%) after 10 times of recycling as well as after storage at elevated temperatures (37 °C for 10 days). With these three outstanding properties, recyclable fluorescent probes can be designed to detect various biomarkers of clinical importance by using different enzymes.
    Publicationtype: Journal Article
      5