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Item type:Publication, Access status: without files , Kultivierung von Mikroorganismen: Experimente aus der KisteExperimental learning is indispensable for the understanding of (natural) scientific concepts. In order to counteract the didactic, methodological and structural challenges of experimentation in everyday school life, a concept for experiments from the “Experimentierkiste” is presented. Thematically, biotechnological approaches with relevance to everyday life are focused on for inquiry-based STEM teaching. The growth and conditions of microorganisms are examined in detail and demonstrated in experiments with yeast and microalgae. The relevance of this topic is based on the socially relevant scientific significance of microbial processes, especially in the medical and biotechnological fields. The core concept of the experimental kit is an essential component for the implementation of such inquiry-based topics with experiments in science lessons. On the topic of yeast, there is a free hands-on experiment website www.kniffelix.de for individuals and school classes from 3rd–13th grade which enhances the learning experience.Publicationtype: Journal Article31 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Beneficial applications of biofilms(Nature Publishing Group, 2024-05); ; Many microorganisms live in the form of a biofilm. Although they are feared in the medical sector, biofilms that are composed of non-pathogenic organisms can be highly beneficial in many applications, including the production of bulk and fine chemicals. Biofilm systems are natural retentostats in which the biocatalysts can adapt and optimize their metabolism to different conditions over time. The adherent nature of biofilms allows them to be used in continuous systems in which the hydraulic retention time is much shorter than the doubling time of the biocatalysts. Moreover, the resilience of organisms growing in biofilms, together with the potential of uncoupling growth from catalytic activity, offers a wide range of opportunities. The ability to work with continuous systems using a potentially self-advancing whole-cell biocatalyst is attracting interest from a range of disciplines, from applied microbiology to materials science and from bioengineering to process engineering. The field of beneficial biofilms is rapidly evolving, with an increasing number of applications being explored, and the surge in demand for sustainable and biobased solutions and processes is accelerating advances in the field. This Review provides an overview of the research topics, challenges, applications and future directions in beneficial and applied biofilm research.Publicationtype: Review Article30 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Enzymatic Oxidation of Butane to 2-Butanol in a Bubble Column(Wiley, 2020-06-09); ; ; ; Unspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non-activated C−H bonds using only hydrogen peroxide as a co-substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we demonstrate the application of a peroxygenase for the hydroxylation of the inert, gaseous substrate butane to 2-butanol in a bubble column reactor. The influence of hydrogen peroxide feed rate and enzyme loading on product formation, overoxidation to butanone and catalytic efficiency is investigated at 200 mL scale. The process is scaled up to 2 L and coupled with continuous extraction. This setup allowed the production of 115 mmol 2-butanol and 70 mmol butanone with an overall total turnover number (TTN) of over 15.000, thereby demonstrating the applicability of peroxygenases for preparative hydroxylation of such inert, gaseous substrates at mild reaction conditions.Publicationtype: Journal ArticleScopus© Citations 11 494 304 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Addition of riboflavin-coupled magnetic beads increases current production in bioelectrochemical systems via the increased formation of anode-biofilms(2019-02-05); ; ; ; Shewanella oneidensis is one of the best-understood model organisms for extracellular electron transfer. Endogenously produced and exported flavin molecules seem to play an important role in this process and mediate the connection between respiratory enzymes on the cell surface and the insoluble substrate by acting as electron shuttle and cytochrome-bound cofactor. Consequently, the addition of riboflavin to a bioelectrochemical system (BES) containing S. oneidensis cells as biocatalyst leads to a strong current increase. Still, an external application of riboflavin to increase current production in continuously operating BESs does not seem to be applicable due to the constant washout of the soluble flavin compound. In this study, we developed a recyclable electron shuttle to overcome the limitation of mediator addition to BES. Riboflavin was coupled to magnetic beads that can easily be recycled from the medium. The effect on current production and cell distribution in a BES as well as the recovery rate and the stability of the beads was investigated. The addition of synthesized beads leads to a more than twofold higher current production, which was likely caused by increased biofilm production. Moreover, 90% of the flavin-coupled beads could be recovered from the BESs using a magnetic separator.Publicationtype: Journal ArticleScopus© Citations 15 91 150