Now showing1 - 1 of 1
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Using planktonic microorganisms to supply the unpurified multi-copper oxidases laccase and copper efflux oxidases at a biofuel cell cathode(Elsevier, 2014-02-17); ; ; ; The feasibility to apply crude culture supernatants that contain the multicopper oxidases laccase or copper efflux oxidase (CueO) as oxygen reducing catalysts in a biofuel cell cathode is shown. As enzyme-secreting recombinant planktonic microorganisms, the yeast Yarrowia lipolytica and the bacterium Escherichia coli were investigated. The cultivation and operation conditions (choice of medium, pH) had distinct effects on the electro-catalytic performance. The highest current density of 119±23μAcm-2 at 0.400V vs. NHE was obtained with the crude culture supernatant of E. coli cells overexpressing CueO and tested at pH 5.0. In comparison, at pH 7.4 the electrode potential at 100μAcm-2 is 0.25V lower. Laccase-containing supernatants of Y. lipolytica yielded a maximum current density of 6.7±0.4μAcm-2 at 0.644V vs. NHE. These results open future possibilities to circumvent elaborate enzyme purification procedures and realize cost effective and easy-to-operate enzymatic biofuel cells.Publicationtype: Journal ArticleScopus© Citations 12 19