Technical Microbiology V-7

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Training Cupriavidus necator for growth on cathode surfaces: impact of adaptive evolution for genomic alterations leading to accelerated biofilm development
(2026-09-23)
Chavali, Sri Sannihita
Cupriavidus necator is a highly adaptable chemolithoautotroph and a promising chassis for sustainable bioproduction from CO₂. Coupling its metabolism to renewable electricity through microbial electrosynthesis (MES) requires the organism to form stable, electroactive biofilms on cathodes. This step is hindered under oxic conditions (oxic MES, oMES) by electrochemically generated reactive oxygen species (ROS), mass-transfer limitations and poor biofilm stability. Systematic strategies to improve C. necator biofilm formation on cathodes have been lacking. Here we used adaptive laboratory evolution (ALE) to address this gap. The progenitor strain colonised graphite cathodes only after an initial heterotrophic phase and formed spatially uneven biofilms whose distribution mirrored the local flow field, as resolved by micro-particle tracking velocimetry. Populations were then selected over five passages under controlled, oxygen-limited cathodic conditions. Resequencing revealed that the recovered population was genetically heterogeneous, and its dominant isolate, strain 890, was characterised in a microfluidic oMES platform. Strain 890 reached the target electrode coverage in about one day instead of six and maintained a robust biofilm at the progenitor's steady-state biovolume, whereas the multi-strain pool peaked higher but proved unstable. Whole-genome sequencing linked this accelerated, biofilm-forward phenotype to only two loci, both potentially central regulators of the planktonic-to-biofilm transition. The steady-state biovolume itself remained bounded most probably due to a mass-transfer ceiling set by the counter-directional supply of H₂ and O₂. This work shows that ALE renders the initial-attachment barrier of cathodic biofilm formation genetically tractable, providing strain 890 as a robust chassis and a well-defined starting point for oMES-based CO₂ valorisation.
Data Type: Dataset
10 40
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Identification of genetic determinants that promote biofilm growth under heterotrophic conditions in Cupriavidus necator using transposon enrichment
(2026-07-06)
Weiler, Janek
These datasets contain measurements of OCT and a crystal violet assay to compare biofilm formation of two different Cupriavidus necator strains.
Data Type: Experimental Data
17 31
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Dataset for "Oxygen-dependent redox control enables growth-decoupled biotransformations in Cupriavidus necator"
(2026-04-07)
Jesenofsky, Vivien
This dataset contains measurements from growth and cell suspension experiments using engineered Cupriavidus necator strains for oxygen-dependent biotransformations. Experiments include: Acetoin reduction to 2,3-butanediol under ±O₂; Glycerol conversion to 1,3-propanediol under ±O₂; Growth experiments under oxic conditions; Long-term viability assays with live/dead staining. Oxygen availability was used as a control parameter to modulate intracellular redox metabolism and direct NADH utilization toward either product formation or respiration.
Data Type: Dataset
102 57
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Aerographite vs. Graphite: Investigating the Role of Electrode Architecture in H₂-Mediated Electroautotrophic Biofilms
(2026-03-05)
Chavali, Sri Sannihita
This study investigates biofilm development by 𝘊𝘶𝘱𝘳𝘪𝘢𝘷𝘪𝘥𝘶𝘴 𝘯𝘦𝘤𝘢𝘵𝘰𝘳 H16 on Aerographite, an ultralow-density carbon foam (3-6 mg cm⁻³, porosity >99%) with a hierarchical tetrapodal pore network, as a cathode material in microfluidic bioelectrochemical systems and compares it to plain graphite.
Data Type: Dataset
75 134
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Dissertation René Wurst - Ergänzendes Videomaterial zum Auf- und Umbau dynamischer mikrostrukturierter 3D-Elektroden
(2025-03-18)
Wurst, René
Ergänzendes Videomaterial zum Auf- und Umbau dynamischer mikrostrukturierter 3D-Elektroden. Weitere Informationen können der Dissertation von René Wurst entnommen werden.
Data Type: Video
41 146
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Dissertation Melanie Tabea Knoll - Appendix 6.4: Detachment of biomaterial - OCT and video time lapse
(2023-10-13)
Knoll, Melanie Tabea
In bioelectrochemical systems (BES), the conversion of chemical energy into electrical energy and vice versa can be catalyzed by electroactive microorganisms. The microorganism-electrode interaction is a key factor that can limit sufficient space-time yield required for industrial applications. Providing the organisms with an artificial scaffold that enhances this interaction compared to the naturally formed biofilm matrix can significantly improve the productivity of these systems. In the context of the dissertation, such a hybrid biomaterial was established by embedding the electroactive model organism Shewanella oneidensis in an agarose hydrogel. The possibility of detaching the hybrid biofilm material should be investigated, as the recovery of the biomaterial could be essential in future application processes and reveal the maximum number of electrons that can be supplied before the material degrades. The latter is an important parameter for the potential application of the biomaterial in bioelectrosynthesis, a process in which organisms grow on the cathode as a source of electrons and energy and where the biomaterial could have similar beneficial effects on productivity to those shown in this thesis for anodic systems. Synthetic biofilm detachment was induced by applying a negative current to a sprayed hydrogel in a BES flow cell reactor. Detachment from two electrode materials, a graphite plate and a graphite felt, was visualized using video recording and optical coherence tomography (OCT). The graphite felt resulted in partial detachment of parts of the biofilm, whereas the graphite plate resulted in complete detachment of the biomaterial as a whole.
Data Type: Video
180 68

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