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    Item type:Research Data, Access status: with files ,
    M03.004 Determination of fatty acid methyl ester (FAME) by GC-FID
    (2016-07-11)
    Simon, Andrea
    ;
    Stahl, Alina
    Fatty acids are the major components of lipids and oils. The properties of lipid classes depend primarily on their fatty acid composition. Due to its high molecular mass and resulting low volatility, fatty acid composition is determined as the methyl esters of fatty acids (FAME) by gas-liquid chromatography with FID detector. FAMES are produced by transesterification between triglycerides and methanol in the presence of sodium hydroxide. These methyl esters are solved in hexane and analysed with GC-FID. Evaluation is based on external calibration, results are reported in g/kg or percent by weight.
    Data Type: Text
      339  389
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    Item type:Research Data, Access status: with files ,
    M03.006 Content of free fat by Soxhlet extraction
    (2016-07-05)
    Romann, Eike Merten
    ;
    Stahl, Alina
    ;
    Simon, Andrea
    The content of free fat in samples is determined by solvent extraction. Therefore, a homogenised amount of sample is extracted by boiling it with petroleum benzine (boiling range 40-60°C) for six hours in a Soxhlet apparatus. After evaporation of solvent, the content of fat in sample is determined gravimetrically.
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      295  149