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Item type:Publication, Access status: without files , Rapid Response to Pandemic Threats: Immunogenic Epitope Detection of Pandemic Pathogens for Diagnostics and Vaccine Development Using Peptide Microarrays(2020-11-06); ; ; Emergence and re-emergence of pathogens bearing the risk of becoming a pandemic threat are on the rise. Increased travel and trade, growing population density, changes in urbanization, and climate have a critical impact on infectious disease spread. Currently, the world is confronted with the emergence of a novel coronavirus SARS-CoV-2, responsible for yet more than 800 »000 deaths globally. Outbreaks caused by viruses, such as SARS-CoV-2, HIV, Ebola, influenza, and Zika, have increased over the past decade, underlining the need for a rapid development of diagnostics and vaccines. Hence, the rational identification of biomarkers for diagnostic measures on the one hand, and antigenic targets for vaccine development on the other, are of utmost importance. Peptide microarrays can display large numbers of putative target proteins translated into overlapping linear (and cyclic) peptides for a multiplexed, high-throughput antibody analysis. This enabled for example the identification of discriminant/diagnostic epitopes in Zika or influenza and mapping epitope evolution in natural infections versus vaccinations. In this review, we highlight synthesis platforms that facilitate fast and flexible generation of high-density peptide microarrays. We further outline the multifaceted applications of these peptide array platforms for the development of serological tests and vaccines to quickly encounter pandemic threats.Publicationtype: Review Article11 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Active pneumatic microballoon mixing on centrifugal microfluidic platforms(2020-01-01); ; ; Perebikovsky, AlexandraIn this study, we are introducing an liquid reciprocation technique on centrifugal microfluidic platforms based on active microballoons. The liquid reciprocation technique is tested for mixing 22 µl water with 3 µl color in a large-surface-area-to-volume-ratio chamber. The color homogenization within the shallow chamber was achieved in less than 5 seconds, during which about sixty liquid reciprocation cycles were accomplished. As an early proof of concept for using the active flow reciprocation mechanism and peptide microarrays in point of care (POC) diagnostics applications, we co-integrated both technologies in a microfluidic disc for detection of 15 nM anti-FLAG Cy5 antibodies.Publicationtype: Conference Paper7 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Elastic reversible valves on centrifugal microfluidic platforms(2019-03-21); 3 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Antibody fingerprints in lyme disease deciphered with high density peptide arrays(2017-10-01); ; ; 6 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution(2017-10-01); ; ; ; Vaccinations are among the most potent tools to fight infectious diseases. However, cross-reactions are an ongoing problem and there is an urgent need to fully understand the mechanisms of the immune response. For the development of a methodological workflow, the linear epitopes in the immune response to the tetanus toxin is investigated in sera of 19 vaccinated Europeans applying epitope mapping with peptide arrays. The most prominent epitope, appearing in nine different sera (923IHLVNNESSEVIVHK937), is investigated in a substitution analysis to identify the amino acids that are crucial for the binding of the corresponding antibody species − the antibody fingerprint. The antibody fingerprints of different individuals are compared and found to be strongly conserved (929ExxEVIVxK937), which is astonishing considering the randomness of their development. Additionally, the corresponding antibody species is isolated from one serum with batch chromatography using the amino acid sequence of the identified epitope and the tetanus specificity of the isolated antibody is verified by ELISA. Studying antibody fingerprints with peptide arrays should be transferable to any kind of humoral immune response toward protein antigens. Furthermore, antibody fingerprints have shown to be highly disease-specific and, therefore, can be employed as reliable biomarkers enabling the study of cross-reacting antigens.Publicationtype: Journal Article6 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Selective Functionalization of Microstructured Surfaces by Laser-Assisted Particle TransferMicrocavity arrays represent millions of different reaction compartments to screen, for example, molecular interactions, exogenous factors for cells or enzymatic activity. A novel method is presented to selectively synthesize different compounds in arrays of microcavities with up to 1 000 000 cavities per cm<sup>2</sup>. In this approach, polymer microparticles with embedded pre-activated monomers are selectively transferred into microcavities with laser radiation. After particle patterning, heating of the particle matrix simultaneously leads to diffusion and coupling of the monomers inside each microcavity separately. This method exhibits flexibility, not only in the choice of compounds, but also in the choice of particle matrix material, which determines the chemical reaction environment. The laser-assisted selective functionalization of microcavities can be easily combined with the intensively growing number of laser applications for patterning of molecules and cells, which is useful for the development of novel biological assays.Publicationtype: Journal Article1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material(2016-06-14); ; ; ; Schlageter, MartinLaser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array's peptides with >17,000 spots per cm2Publicationtype: Journal Article13 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Robust recognition of micro-sized fiducial marks combining position and identification information(VDE Verlag, 2015-10); 5