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Item type:Publication, Access status: without files , Spatial modes of laser-induced mass transfer in micro-gaps(2019-04-01); ; ; We have observed the concentric deposition patterns of small molecules transferred by means of laser-induced forward transfer (LIFT). The patterns comprised different parts whose presence changed with the experimental constraints in a mode-like fashion. In experiments, we studied this previously unknown phenomenon and derived model assumptions for its emergence. We identified aerosol micro-flow and geometric confinement as the mechanism behind the mass transfer and the cause of the concentric patterns. We validated our model using a simulation.Publicationtype: Journal Article1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Combinatorial Synthesis of Macromolecular Arrays by Microchannel Cantilever Spotting (µCS)(2018-08-02); ; Surface-bound microarrays of multiple oligo- and macromolecules (e.g., peptides, DNA) offer versatile options in biomedical applications like drug screening, DNA analysis, or medical diagnostics. Combinatorial syntheses of these molecules in situ can save significant resources in regard to processing time and material use. Furthermore, high feature densities are needed to enable high-throughput and low sample volumes as generally regarded in combinatorial chemistry. Here, a scanning-probe-lithography-based approach for the combinatorial in situ synthesis of macromolecules is presented in microarray format. Feature sizes below 40 µm allow for the creation of high-density arrays with feature densities of 62 500 features per cm<sup>2</sup>. To demonstrate feasibility of this approach for biomedical applications, a multiplexed array of functional protein tags (HA- and FLAG-tag) is synthesized, and selective binding of respective epitope recognizing antibodies is shown. This approach uses only small amounts of base chemicals for synthesis and can be further parallelized, therefore, opening up a route to flexible, highly dense, and cost-effective microarrays.Publicationtype: Journal Article3 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Combinatorial Particle Patterning(2017-11-10); ; ; The unique properties of solid particles make them a promising element of micro- and nanostructure technologies. Solid particles can be used as building blocks for micro and nanostructures, carriers of monomers, or catalysts. The possibility of patterning different kinds of particles on the same substrate opens the pathway for novel combinatorial designs and novel technologies. One of the examples of such technologies is the synthesis of peptide arrays with amino acid particles. This review examines the known methods of combinatorial particle patterning via static electrical and magnetic fields, laser radiation, patterning by synthesis, and particle patterning via chemically modified or microstructured surfaces.Publicationtype: Review Article5 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Selective Functionalization of Microstructured Surfaces by Laser-Assisted Particle TransferMicrocavity arrays represent millions of different reaction compartments to screen, for example, molecular interactions, exogenous factors for cells or enzymatic activity. A novel method is presented to selectively synthesize different compounds in arrays of microcavities with up to 1 000 000 cavities per cm<sup>2</sup>. In this approach, polymer microparticles with embedded pre-activated monomers are selectively transferred into microcavities with laser radiation. After particle patterning, heating of the particle matrix simultaneously leads to diffusion and coupling of the monomers inside each microcavity separately. This method exhibits flexibility, not only in the choice of compounds, but also in the choice of particle matrix material, which determines the chemical reaction environment. The laser-assisted selective functionalization of microcavities can be easily combined with the intensively growing number of laser applications for patterning of molecules and cells, which is useful for the development of novel biological assays.Publicationtype: Journal Article1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material(2016-06-14); ; ; ; Schlageter, MartinLaser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array's peptides with >17,000 spots per cm2Publicationtype: Journal Article13 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Robust recognition of micro-sized fiducial marks combining position and identification information(VDE Verlag, 2015-10); 5