Concentration Data of the Aldol Reaction Catalyzed by L-threonine Aldolases Measured by In-Line NMR

The dataset contains concentration data from the aldol reaction between benzaldehyde and glycine, catalyzed by L-threonine aldolase, yielding L-threo- and L-erythro-β-phenylserine. Measurements were performed using benchtop NMR and are used for progress curve analysis in the context of a scientific publication. The data are provided as supplementary information to this publication.

Content

Subjects

Concentrations

in-line data

biocatalysis

DDC Class

660.6: Biotechnology

Funding References

Funding(s)

Überwindung der Limitierungen von L-Threoninaldolasen in der Biokatalyse: Durch Reaktionstechnik zu hocheffizienten Anwendungen

Funding Organisations

Deutsche Forschungsgemeinschaft (DFG)

Files

License

https://creativecommons.org/publicdomain/zero/1.0/

Technical information

Methods for processing the data:
Spectra were processed with PEAXACT 5.7 (baseline subtraction, phase correction, normalization) and analyzed via Complemental Hard Modeling. Resulting concentrations were extracted and organized in Excel format.

Instrument- or software-specific information needed to interpret the data:
Spinsolve 80 Carbon ULTRA (Magritek), PEAXACT 5.7, Python 3.11.9 with SciPy (odeint, differential_evolution)

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Name

Concentration_Data.zip

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117.46 KB

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ZIP

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Name

Read Me.txt

Size

5.06 KB

Format

Text

Download

Name

InitialRate_Glycine_Varied_50mM_Benzaldehyde.xlsx

Size

197.2 KB

Format

Microsoft Excel XML

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Name

InitialRate_ThreoPhenylserine_Varied.xlsx

Size

199.03 KB

Format

Microsoft Excel XML

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Name

InitialRate_Benzaldehyde_Varied_1000mM_Glycine.xlsx

Size

224.15 KB

Format

Microsoft Excel XML