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Item type:Publication, Access status: without files , Synthetic High-Throughput Microarrays of Peptidoglycan Fragments as a Novel Sero-Diagnostic Tool for Patient Antibody Profiling(2025-04-25); ; ; ; Peptidoglycan (PGN) is a complex biopolymer crucial for cell wall integrity and function of all bacterial species. While the strong inflammatory properties of PGN and its derived muropeptides are well-documented in human innate immune responses, adaptive immunity, including antibody responses to PGN, remain inadequately characterized. Microarray technology represents a cost- and time-efficient method for studying such interactions. Our laser-based technology enables the high-throughput synthesis of biomolecules on functionalized glass slides. Here, this on-chip synthesis was developed for PGN fragments, to generate a variety of 216 stem peptides and attach six different glycan moieties that are major structural components of bacterial cell walls. Thereby, 864 PGN fragments from different Gram-negative and Gram-positive species were generated. The arrays were validated with four different monoclonal antibodies against PGN or poly-N-acetyl glucosamine and identified their epitopes. Finally, proof of concept for antibody profiling in patient samples was performed by comparing a panel of well-characterized plasma samples of epidermolysis bullosa (EB) patients suffering from (chronic) wounds with Staphylococcus aureus infection. EB patients show an increased response to the muramyl dipeptide. Therefore, this novel high-throughput PGN glycopeptide microarray technology promises to identify distinct antibody profiles against human microbiomes in diseases, notably in those involving the intestine.Publicationtype: Journal Article21 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: with files , Synthetische Hochdurchsatz‐Mikroarrays von Peptidoglykan Fragmenten als neuartiges serodiagnostisches Instrument zur Erstellung von Antikörperprofilen bei Patienten(Wiley, 2025-02-28); ; ; ; Peptidoglykane (PGN) sind komplexe Biopolymere, die für die Integrität und Funktion der Zellwand aller Bakterienarten essenziell sind. Während die entzündungsfördernden Eigenschaften von PGN und seinen Muropeptiden in der angeborenen Immunreaktion weithin bekannt sind, ist die adaptive Immunität, insbesondere die Antikörperreaktionen auf PGN, noch wenig erforscht. Die Microarray-Technologie stellt eine kosten- und zeiteffiziente Methode zur Untersuchung solcher Interaktionen dar. Unsere Lasertechnologie ermöglicht die Hochdurchsatzsynthese von Biomolekülen auf funktionalisierten Glasträgern. Hier wurde diese On-Chip-Synthese für PGN-Fragmente weiterentwickelt, um 216 verschiedene Stammpeptide herzustellen und sechs verschiedene Glykaneinheiten, die wichtige Strukturkomponenten bakterieller Zellwände sind, anzukuppeln. Auf diese Weise wurden 864 PGN-Fragmente aus verschiedenen Gram-negativen und Gram-positiven Spezies hergestellt. Die Arrays wurden mit vier verschiedenen monoklonalen Antikörpern gegen PGN oder Poly-N-Acetylglucosamin und die Identifizierung ihrer Epitope überprüft. Schließlich wurde ein Konzept für die Erstellung von Antikörperprofilen in Patientenproben demonstriert. Hierzu wurde ein Panel von gut charakterisierten Plasmaproben von Patienten mit Epidermolysis bullosa (EB) mit (chronischen) Wunden, die mit Staphylococcus aureus infiziert waren, verglichen. EB-Patienten zeigen eine erhöhte Reaktion auf das Muramyl-Dipeptid. Diese neue Hochdurchsatz-PGN-Glykopeptid-Microarray-Technologie ermöglicht somit die Identifizierung von potentiell verschiedenen Antikörperprofilen gegen das menschliche Mikrobiom bei Krankheiten, insbesondere bei Darmerkrankungen.Publicationtype: Journal Article40 38 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , MVA-based vaccine candidates encoding the native or prefusion-stabilized SARS-CoV-2 spike reveal differential immunogenicity in humans(2024-12-01); ; 10 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Printed smart devices for anti-counterfeiting allowing precise identification with household equipment(2024-12-01); ; Counterfeiting has become a serious global problem, causing worldwide losses and disrupting the normal order of society. Physical unclonable functions are promising hardware-based cryptographic primitives, especially those generated by chemical processes showing a massive challenge-response pair space. However, current chemical-based physical unclonable function devices typically require complex fabrication processes or sophisticated characterization methods with only binary (bit) keys, limiting their practical applications and security properties. Here, we report a flexible laser printing method to synthesize unclonable electronics with high randomness, uniqueness, and repeatability. Hexadecimal resistive keys and binary optical keys can be obtained by the challenge with an ohmmeter and an optical microscope. These readout methods not only make the identification process available to general end users without professional expertise, but also guarantee device complexity and data capacity. An adopted open-source deep learning model guarantees precise identification with high reliability. The electrodes and connection wires are directly printed during laser writing, which allows electronics with different structures to be realized through free design. Meanwhile, the electronics exhibit excellent mechanical and thermal stability. The high physical unclonable function performance and the widely accessible readout methods, together with the flexibility and stability, make this synthesis strategy extremely attractive for practical applications.Publicationtype: Journal Article14 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Confined Flash Printing and Synthesis of Stable Perovskite Nanofilms under Ambient Conditions(2024-11-14); ; ; The fabrication of stable perovskite nanofilm patterns is important for the development of functional optical devices. However, current production approaches are limited by the requirement for strict inert gas protection and long processing times. Here, a confined flash printing synthesis method is presented to generate perovskite nanofilms under ambient conditions, combining precursor transfer, perovskite synthesis, crystallization, and polymer protection in a single step within milliseconds. A laser simultaneously prints and induces the flash synthesis, confined in a polymer nanofilm, under normal ambient conditions. Due to its simplicity and flexibility, the method enables the combination and screening of many different perovskite precursor materials on various substrates. Besides for the development of novel perovskite materials and devices, the nanofilms can be applied for biodetection. The unique H<inf>2</inf>O<inf>2</inf>-responsive property of the ultrathin perovskite quantum dot film is applied for biomolecule detection based on oxidase-catalyzed enzymatic reactions.Publicationtype: Journal Article11 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , VaporLIFT: On-Chip Chemical Synthesis of Glycan Microarrays(2024-07-03); ; ; ; Garcia Ricardo, ManuelLaser-induced forward transfer (LIFT) of polymers is a versatile printing method for parallel in situ synthesis of peptides on microarrays. Chemical building blocks embedded in a polymer matrix are transferred and coupled in a desired pattern to a surface, generating peptides on microarrays by repetitive in situ solid-phase synthesis steps. To date, the approach is limited to simple, heat induced chemical reactions. The VaporLIFT method, disclosed here, combines LIFT with chemical vapor glycosylation to rapidly generate glycans on microarray surfaces while maintaining inert, low temperature conditions required for glycosylations. Process design and parameter optimization enables the synthesis of a collection of glycans at defined positions on a glass surface. The synthetic structures are detected by mass spectrometry, fluorescently labeled glycan-binding proteins, and covalent staining with fluorescent dyes. VaporLIFT is ideal for parallel screening of other chemical reactions, that require inert and well-defined reaction conditions.Publicationtype: Journal Article17 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Redox-responsive inorganic fluorescent nanoprobes for serodiagnosis and bioimaging(2024-06-15); ; ; Redox reactions play fundamental roles in life and are at the core of metabolism. Thus, observing and quantifying these reactions is crucial for diagnostics and therapy. Recent advances in inorganic fluorescent nanoprobes have revolutionized the field, enabling in vitro diagnostics by providing reliable tools for real-time, quantitative determination of redox biomolecule levels in biological samples and cells. Due to their high stability, these probes are also widely used in bioimaging, providing real-time information for in vivo diagnostics and guiding treatment of diseases associated with redox biomolecules. This review explores the diverse landscape of inorganic fluorescent nanoprobes designed for the detection of biologically relevant reactive oxygen and nitrogen species. The discussion is divided into several sections, each focusing on nanoprobes tailored for specific oxidative species. The impact of tailored nanoprobes in diagnostics and imaging-guided treatment depends on their chemical composition, surface property, and fluorescence mechanism. The discussions highlight the current strengths and weaknesses, which will help to design more efficient redox-responsive inorganic fluorescent nanoprobes in the future.Publicationtype: Review Article9 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Customized Enhancement of Thermal Sensitivity of Tumors at Different Subcutaneous Depths by Multichannel Lanthanide Nanocomposites(2024-06-06); ; 9 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Sustainable design of high-performance multifunctional carbon electrodes by one-step laser carbonization for supercapacitors and dopamine sensors(2024-04-09); ; ; ; Laser carbonization is a rapid method to produce functional carbon materials for electronic devices, but many typical carbon precursors are not sustainable and/or require extensive processing for electrochemical applications. Here, a sustainable concept to fabricate laser patterned carbon (LP-C) electrodes from biomass-derived sodium lignosulfonate, an abundant waste product from the paper industry is presented. By introducing an adhesive polymer interlayer between the sodium lignosulfonate and a graphite foil current collector, stable, abrasion-resistant LP-C electrodes can be fabricated in a single laser irradiation step. The electrode properties can be systematically tuned by controlling the laser processing parameters. The optimized LP-C electrodes demonstrate a promising performance in supercapacitors and electrochemical dopamine biosensors. They exhibit high areal capacitances of 38.9 mF cm−2 in 1 M H2SO4 and high energy and power densities of 4.3 μW h cm−2 and 16 mW cm−2 in 17 M NaClO4, showing the best performance among biomass-derived LP-C materials reported so far. After 20 000 charge/discharge cycles, they retain a high capacitance of 81%. Dopamine was linearly detected in the range of 0.1 to 20 μM with an extrapolated limit of detection of 0.5 μM (S/N = 3) and high sensitivity (13.38 μA μM−1 cm−2), demonstrating better performance than previously reported biomass-derived LP-C dopamine sensors.Publicationtype: Journal Article5 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Access status: without files , Flash phase engineering of MoS2 nanofilms for enhanced photoelectrochemical performance(2024-02-05); 12